Practical considerations for the study of the oral microbiome
À¯¿¬ÀÌ, À̼¿µ, ³ªÈñ»ï,
¼Ò¼Ó »ó¼¼Á¤º¸
À¯¿¬ÀÌ ( Yu Yeun-I ) - Pusan National University Interdisplinary Program of Genomic Science
À̼¿µ ( Lee Seo-Young ) - Pusan National University Interdisplinary Program of Genomic Science
³ªÈñ»ï ( Na Hee-Sam ) - Pusan National University School of Dentistry Department of Oral Microbiology
Abstract
In the oral cavity, complex microbial community is shaped by various host and environmental factors. Extensive literature describing the oral microbiome in the context of oral health and disease is available. Advances in DNA sequencing technologies and data analysis have drastically improved the analysis of the oral microbiome. For microbiome study, bacterial 16S ribosomal RNA gene amplification and sequencing is often employed owing to the cost-effective and fast nature of the method. In this review, practical considerations for performing a microbiome study, including experimental design, molecular analysis technology, and general data analysis, will be discussed.
Å°¿öµå
Oral microbiome; 16S rDNA; Bacterial associations; High-throughput nucleotide sequencing
¿ø¹® ¹× ¸µÅ©¾Æ¿ô Á¤º¸
µîÀçÀú³Î Á¤º¸